Table 2.
Activities of WT and Y172A CgAM enzymes
Reaction | Sp acta (U/mg protein) |
|
---|---|---|
WT CgAM | Y172A CgAM | |
Disproportionation | 21.8 ± 0.59 | 6.18 ± 0.05 |
Hydrolysis | 8.05 ± 0.04 | 4.22 ± 0.02 |
Cyclization | 0.502 ± 0.06 | 0.283 ± 0.03 |
Coupling | NDb | ND |
Data are presented as the mean ± standard deviation and are derived from three independent repeats.
ND, not detectable.