Table 3.
Kinetic parameters of WT and Y172A CgAM enzymes derived from the disproportionation reaction using maltotriose as the substratea
| CgAM enzyme | Km (mM) | kcat (min−1 [103]) | kcat/Km (mM−1min−1 [103]) |
|---|---|---|---|
| Wild type | 19.6 ± 3.2 | 9.37 ± 1.1 | 0.479 ± 0.1 |
| Y172A mutated | 12.9 ± 0.8 | 2.17 ± 0.2 | 0.168 ± 0.1 |
a
Data shown are the mean ± standard deviation and are derived from Lineweaver-Burk plots of the results of three independent sets of different enzyme-substrate concentrations with product analysis at different time points.