Table 1.
Oligonucleotide primers used for the detection and quantification of chicken/turkey parvoviruses
| Primers | Genome regiona | Positionb | Amplification reaction | Amplicon size (bp) | Annealing temp (°C) | Sequence (5′–3′) |
|---|---|---|---|---|---|---|
| Par1 | NS | 661–682 | First | 412 | 53 | GGTACAAGATATGCTAGATTTG |
| Par2 | 1053–1073 | CGGATGGCTAAATTATCATCT | ||||
| Par3 | 718–739 | Nested | 325 | 53 | CCATCGCAGGAATTAACTCCAG | |
| Par4 | 1022–1043 | GTGTCAACATCTCCATGTATTG | ||||
| VP-Par1 | VP1/VP2 | 3119–3140 | First | 373 | 56 | TGGAATTGTGATACTATATGGG |
| VP-Par2 | 3473–3492 | TCYTGATCTGCAAATATTTG | ||||
| VP-Par3 | 3173–3196 | Nested | 249 | 64 | CATTGTGTCTGTCTWATGCGTGAC | |
| VP-Par4 | 3405–3422 | GTTTTCTGGATGACTTGCA | ||||
| Q-PaV-F | VP1/VP2 | 3326–3345 | qPCR | 81 | 60 | AGTCCACGAGATTGGCAACA |
| Q-PaV-R | 3388–3407 | GCAGGTTAAAGATTTTCACG | ||||
| Q-PaV-Pr | 3356–3378 | 6FAM-AATTATTCGAGATGGCGCCCACG-BHQ1 |
a
NS, nonstructural protein 1; VP1, virion protein 1; VP2, virion protein 2.
b
The sequence positions are with reference to accession number GU214706 from GenBank.