Fig 1.

Purification of female-specific lectin AOL1 from Aglaothamnion oosumiense by the use of agarose-bound GalNAc affinity chromatography. (A) SDS-PAGE of eluted proteins collected from GalNAc-agarose column. Lanes 1 to 13, gel electrophoresis according to the order of eluted fractions 1 through 13. The arrow points to the protein bands with a molecular mass of ca. 22 kDa. (B) SDS-PAGE of samples according to purification step. Lane 1, crude extract; lane 2, washing fraction; lane 3, purified AOL1. M, molecular mass markers.