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Thermostability of activities and structures of recombinant enzymes. The β-glucosidase activities of recombinant Gluc1C (A) and EG5 (B) were tested at various temperatures with respect to time. (C) The stability of secondary structures of Gluc1C, Endo5A, and the EG5 chimera was determined by denaturing the recombinant enzymes with increasing temperature and measuring the CD signals at 220 nm.
