Table 4.

In silico analysis of parameters affecting the structure of β-glucosidase in EG5 chimera

Parametera	Result for enzyme:		% increase in EG5
	Gluc1C	EG5
Frac-nonpol buried area	0.26	0.28	7.8
Frac-exp-pol area	0.28	0.31	10.7
Helical content (%)	38.7	40.5	4.6
Hydrophobicity	0.62	0.67	8.1

^a“Frac-nonpol buried area” denotes the contribution of nonpolar residues to the buried surface area, and “Frac-exp-pol area” denotes the contribution of polar residues to the exposed surface area. “Helical content” refers to the percentage of residues that have an α-helical conformation in the protein. Hydrophobicity was calculated as the fraction of the buried nonpolar area out of the total nonpolar area.