Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| XL1 MR | Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrn173 endA1 supE44 thi1 recA1 gyrA96 relA1 lac) | Stratagene |
| R. rubrum | ||
| S1 | Wild type | 7 |
| ST4 | S1-derived crtD mutant; Kanr | 27 |
| SLYC18 | S1-derived crtC crtD site-directed mutant; Kanr | This study |
| Plasmids | ||
| pBsKSII+ | High-copy cloning vector, ColE1; Ampr | Stratagene |
| pBsLGKan | pBsKSII+ derivative, carrying the kanamycin resistance cassette; Kanr | 32 |
| pRK404 | Derivative of pRK290; mob+ | 9 |
| pRK2013 | Mobilizing helper plasmid, tra+; Kanr | 12 |
| pBsSGE5 | pBsKSII+ derivative, containing the 6.37-kb EcoRV fragment from the pSC4 cosmid | This study |
| pBsSGE5K1 | pBsSGE5 derivative, containing the kanamycin resistance cassette in the opposite direction to the crtD gene | This study |
| pBsSGE5K2 | pBsSGE5 derivative, containing the kanamycin resistance cassette in the same direction as the crtD gene | This study |
| pRKSGE5 | pRK404 derivative, containing the 6.42-kb HindIII/XbaI insert from pBsSGE5 | This study |
| pSUP202 | Suicide vector, ColE1, mob+; Ampr Cmr Tetr | 50 |
| pSUPSGE5K1 | pSUP202 derivative, containing the HindIII/SalI insert of pBsSGE5K1, Ampr Kanr Cmr Tetr | This study |
| pSUPSGE5K2 | pSUP202 derivative, containing the HindIII/SalI insert of pBsSGE5K2, Ampr Kanr Cmr Tetr | This study |
a
Cmr, chloramphenicol resistance; Tetr, tetracycline resistance; Ampr, ampicillin resistance; Kanr, kanamycin resistance.