Table 1.
Primers and probes used for qPCR assays
| Target | Primer | Sequence (5′–3′)a | Reference |
|---|---|---|---|
| Human-associated Bacteroides 16S rRNA | HF183 | ATCATGAGTTCACATGTCCG | 7 |
| SSHBacR | TACCCCGCCTACTATCTAATG | 34 | |
| SSHBac-PRB | (FAM)-TTAAAGGTATTTTCCGGTAGACGATGG-(TAMRA) | 19 | |
| HPyV conserved T-antigen | SM2 | AGT CTT TAG GGT CTT CTA CCT TT | 33 |
| P6 | GGT GCC AAC CTA TGG AAC AG | 3 | |
| KGJ3 | (FAM)-TCA TCA CTG GCA AAC AT-(MGBNFQ) | 33 | |
| General Bacteroidales (GenBac) | GenBacF3 | GGGGTTCTGAGAGGAAGGT | 30 |
| GenBacR4 | CCGTCATCCTTCACGCTACT | 10 | |
| GenBacP2 | (FAM)- CAATATTCCTCACTGCTGCCTCCCGTA -(TAMRA) | 10 | |
| IAC | UCP1 | (VIC)-CCTGCCGTCTCGTGCTCCTCA-(TAMRA) | 22 |
a
FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine.