Table 4.

P_LOQ and P_LOD of HPyV and HF183 assays using serially diluted sewage spiked into ambient waters

Site	Datee	HPyV assay			HF183 assay
		PLOQ		PLOD dilutionb	PLOQ		PLOD dilutionb
		No. of target copies · 5 μl−1a	Dilutionb		No. of target copies · 5 μl−1a	Dilutionb
Bahia Beach	9/2/10	3.23 × 102	10−2	10−3	8.31 × 102	10−4	10−6
	9/15/10	1.06 × 101	10−3	10−3	6.63 × 101	10−3	10−3
Fort DeSoto	9/2/10	2.05 × 101	10−4	10−4	8.81 × 101	10−5	10−6
	9/15/10	3.07 × 101	10−3	10−3	9.07 × 101	10−5	10−5
Green Swampc	9/1/10	3.91 × 102	10−2	10−3	1.66 × 102	10−5	10−5
	9/16/10	1.64 × 102	10−2	10−2	1.31 × 101	10−5	10−6
Lake Carroll	9/1/10	1.20 × 102	10−2	10−3	1.98 × 102	10−5	10−6
	9/16/10	6.17 × 101	10−2	10−2	5.23 × 102	10−4	10−4
Hillsborough Riverd	9/2/10	5.47 × 101	10−4	10−4	1.71 × 101	10−6	10−6
	9/16/10	3.11 × 101	10−2	10−4	4.30 × 102	10−4	10−5

^aValues represent the lowest number of target copies per reaction detected at the lowest sewage dilution quantifiable.

^bSewage dilution corresponding to the limit of quantification or detection.

^cResults are estimated due to background contribution of HF183.

^dExtracted DNA was further purified due to detection of inhibition on both sample dates.

^eSample date (month/day/year) corresponds to the collection date for the sewage sample used to spike each ambient water sample.