Table 5.
Correlation analysis of observed values on second sampling date for HF183 compared to a theoretical data set for the sewage spike dilution series
| Sewage dilution factora | Data and correlation analysis for siteb |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fort DeSoto |
Green Swamp |
Lake Carroll |
Hillsborough Riverc |
|||||||||
| Observed quantity | Theoretical quantity | Correlation | Observed quantity | Theoretical quantity | Correlationn | Observed quantity | Theoretical quantity | Correlation | Observed quantity | Theoretical quantity | Correlation | |
| 10−2 | 5.13 | 5.11 | 5.59 | 5.59 | 5.08 | 5.08 | 3.91 | 3.91 | ||||
| 10−3 | 4.66 | 4.11 | 3.64 | 4.59 | 4.08 | 4.08 | 4.05 | 2.91 | ||||
| 10−4 | 3.28 | 3.11 | 2.04 | 3.59 | 3.34 | 3.08 | 3.10 | 1.91 | ||||
| 10−5 | 2.56 | 2.11 | 2.21 | 2.59 | NQd | NQ | NQ | NQ | ||||
| r2 value | 0.97 | 0.85 | 0.99 | 0.62 | ||||||||
| P value | 0.0164 | 0.0785 | 0.0548 | 0.4205 | ||||||||
Dilution factor of 10−2 represents 5 ml sewage inoculated into 500 ml water.
Quantities are expressed as log10 gene copies · 100 ml−1.
DNA extracted from Hillsborough River samples on both sample dates was further purified to relieve inhibition.
NQ, not quantifiable.