Table 2.
Oligonucleotides used for construction of mutants in the 3A protein
| Oligonucleotidea | Sequence (5′→3′)b | Positionb |
|---|---|---|
| 3AF | GAAGATCTCCGACTCGCTCTCCAG | 4199 |
| 3AR | ATCGGACCGGCGTAAGGTCCCTC | 5839 |
| L38E-F | CATTAAGGAGGAAGAGCGGCCCCTCATC | 5397 |
| L38E-R | GATGAGGGGCCGCTCTTCCTCCTTAATG | 5424 |
| L41E-F | GAACTCCGGCCCGAGATCCAACAAAC | 5407 |
| L41E-R | GTTTGTTGGATCTCGGGCCGGAGTTC | 5432 |
| L38EL41E-F | TTAGGAGGAAGAGCGGCCCGAGATCCAA | 5398 |
| L38EL41E-R | TTGGATCTCGGGCCGCTCTTCCTCCTTA | 5437 |
| Q44R-F | CCTCATCCAACGAACTTCATTTGTGAAACGT | 5418 |
| Q44R-R | CACAAATGAAGTTCGTTGGATGAGGGGC | 5442 |
| Q44D-F | CCTCATCCAAGATACTTCATTTGTGAAACGTG | 5418 |
| Q44D-R | CACAAATGAAGTATCTTGGATGAGGGGC | 5442 |
| M29R-F | CTTTGAGGGCCGAGTACACGACTCC | 5373 |
| M29R-R | GGAGTCGTGTACTCGGCCCTCAAAG | 5397 |
a
Forward and reverse primers are indicated by F and R, respectively.
b
Sequences in bold and nucleotide numbering correspond to those of FMDV C-S8c1 (GenBank no. AJ133357), and substituted nucleotides are in italics. Restriction sites are indicated, and their nucleotides are underlined.