Fig 4.

RREs are required for R-dependent BARF1 promoter activation. (A) Site-directed mutations of the RREs in a BARF1 promoter ATG-582 luciferase reporter construct were made. Seven nucleotides, predominantly in the core sequences, were selected for mutation. The original sequences and their respective mutations are shown. (B) AGS cells were transfected with the mutated reporter constructs in combination with either the empty vector or the R expression vector. The amount of luciferase activity was determined 48 h after transfection. The induction of luciferase activity in 3 experiments is shown.