Fig 3.

Ad5 could efficiently infect PBMCs from rhesus macaques with preexisting anti-Ad5 immunity. The macaques were divided into three groups based on the level of preexisting NAb: Ad5 NAb naïve (Nab < 18; n = 9), Ad5 NAb median (18 < NAb titer < 4,608; n = 7), and Ad5 NAb high (NAb titer > 4,608; n = 8). (A) The infected proportion of EGFP⁺ cells; (B) EGFP expression level of the CD14⁺ cell population; (C) the expression levels of SIVenv mRNA in macaque PBMCs were determined by quantitative PCR, as described in Materials and Methods. These data were analyzed statistically by one-way ANOVA followed by Tukey's multiple comparison test. The bars represent the standard errors. *, P < 0.05; **, P < 0.01; ***, P < 0.001.