Fig 4.

Delivery of Ad5-SIVgag through AVIP induced better Gag-specific immune response but less Ad5 NAb than intramuscular injection of Ad5-SIVag in Ad5-seronegative macaques. Twelve macaques, which had been confirmed to be Ad5 seronegative, were divided into three groups (n = 4 per group) as described in Materials and Methods (studyI), and specific immune responses against SIV antigen or Ad5 vector were assessed. (A) SIV-specific cellular immune responses were assessed by IFN-γ ELISPOT assays. (B) NAb against Ad5 vector was detected by Ad5-SEAP-based neutralizing assay, and results represent Ad5 NAb titers. These data were analyzed statistically by two-way ANOVA followed by a Bonferroni post hoc test. The bars represent the standard errors. ***, P < 0.001; ns, no significance.