Fig 5.
Ad5 NAb and Ad5-specific cellular immune responses in Ad5-seropostitive macaques. Fifteen macaques, which had previously been immunized with Ad5-empty vector, were enrolled in studyII. These macaques were divided into three groups in subsequent studies. Preexisting humoral and cellular responses against adenoviral vector were assessed in those macaques. (A) Humoral responses against Ad5. Ad5 NAb was detected by Ad5-SEAP-based neutralizing assay, and results represent Ad5 NAb titers. (B) Cellular responses against Ad5. Macaque PBMCs were stimulated with the lysate of Ad5-empty virus particles, and ELISPOT assays were then performed. The results are represented as spot-forming cells (SFC)/106 PBMCs.