Table 1.
Quantitative RT-PCR analyses of BAFF expression in BMDCs
| Treatment | Relative mRNA expression rate ata: |
|
|---|---|---|
| 0 h | 6 h | |
| Vehicle (0.05% DMSO)b | 0.30 ± 0.04 | 0.40 ± 0.16 |
| CAM (15 μM) | 0.25 ± 0.02 | 0.24 ± 0.07 |
| Poly(I·C) (20 μg/ml) | 0.30 ± 0.10 | 1.47 ± 0.37* |
a
After the cells had been treated with each reagent for 0 and 6 h, total RNA was isolated, and the expression levels of BAFF mRNA normalized to GAPDH mRNA were detected. Data are means ± SEM from four independent experiments. *, P < 0.05 versus vehicle at 6 h.
b
DMSO, dimethyl sulfoxide.