Fig 2.

Cleavage of rRNA by RNase L during EMCV infections and effect of WT and mutant RNase L on autophagy. (A) HeLa M cells stably expressing WT or nuclease-dead mutant (R667A) RNase L were infected with EMCV at MOI of 0.01 for 16 h. Total RNA was isolated and rRNA cleavage was monitored in RNA chips (top panel). LC3B, RNase L, and β-actin were monitored in immunoblots (bottom panels). (B) In a separate experiment, HeLa M cells stably transfected with empty vector (vec) or vector expressing WT RNase L or R667A RNase L were infected with EMCV at MOI of 0.01 for 16 h. P62, RNase L, and β-actin in immunoblots (top) and P62 levels normalized to the β-actin levels (bottom) were determined from three independent biological replicates. Error bars (SD) and P values determined by two-tailed Student's t test (**, P < 0.01) are shown.