Fig 6.

Suppression of autophagy by chemical inhibitors modulates the antiviral activity of RNase L. (A and B) WT and Rnasel^−/− MEF were treated with BafA1 (200 nM) or 3-MA (5 mM) for 2 h prior to infection with EMCV (MOI = 0.05). Viral yields and increases in viral titers were determined from four biological replicates. Error bars, SD; DMSO, dimethyl sulfoxide (solvent control). (C and D) Inhibition of autophagy as described for panel A by BafA1 and 3-MA, respectively, was determined by monitoring P62 and LC3B in immunoblots. (E to G) WT and Rnasel^−/− MEF were treated with BafA1 (200 nM) for 2 h prior to infection with EMCV (MOI = 0.05) for different times. Viral titers (error bars, SD) were determined from three biological replicates. **, P < 0.01; ***, P < 0.001; ns, not significant.