Table 1.
Effects of RNase L and autophagy on viral growtha
| Time (h) | Viral yield (PFU/ml) in: |
Change in viral yield (WT MEF/Rnasel−/− MEF) |
||||||
|---|---|---|---|---|---|---|---|---|
| WT MEF |
Rnasel−/− MEF |
RNase L effect |
Autophagy effect |
|||||
| DMSO (a) | BafA1 (b) | DMSO (c) | BafA1 (d) | −BafA1 (c/a) | +BafA1 (d/b) | WT MEF (b/a) | Rnasel−/− MEF (d/c) | |
| 8 | 3.8 × 105 | 2.7 × 106 | 5.6 × 106 | 9.8 × 106 | 14.7 | 3.6 | 7.1 | 1.75 |
| 12 | 1.7 × 107 | 1.7 × 106 | 1.9 × 108 | 4.5 × 107 | 11.2 | 26.5 | 0.1 | 0.24 |
| 16 | 4.8 × 107 | 3.8 × 106 | 2.6 × 108 | 8.5 × 107 | 5.4 | 22.4 | 0.08 | 0.33 |
a
Results are averages from three biological replicates. Virus titers and statistical parameters are from the experiments whose results are shown in Fig. 6E to G.