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Cross-linking of His-gp20. E. coli BL21 cells expressing His-gp20 were lysed, and the membrane fraction was treated with 0.6% formaldehyde for 30 min. To resolve the cross-links, the sample was incubated at 95°C for 20 min. To identify the protein bands, mass spectrometry was performed and the peptides were identified. 1, DnaK; 2, YidC; 3, GroEL; 4, Tig; 5, EF-Tu.
