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Protease mapping of His-gp20 and His-gp20s. E. coli BL21 cells expressing His-gp20 (A) or His-gp20s (B) were converted to spheroplasts and analyzed on a Western blot (lanes 1). To one aliquot, proteinase K (1 mg/ml) was added for 1 h (lane 2), and for another aliquot, the cells were lysed by 3% Triton X-100 and digested by proteinase K for 1 h (lane 3).
