Fig 1.

Isolation of CoRbs-specific memory B cells by cell sorting. PBMCs from donor 45 were stained with a cocktail containing antibodies for cell surface markers and fluorochrome-labeled Env probes. Cells were gated as indicated: B cells, CD20⁺/CD19⁺; memory B cells, IgG+/IgM−; cells of CD14−/aqua blue−/CD3−/CD8− phenotype were retained, and dead cells (aqua blue+), monocytes (CD14⁺), and T cells (CD3⁺ or CD4⁺/CD8⁺) were gated out; CoRbs-specific memory B cells were gated as gp120 2CC core+/gp140FT I420R− and collected as single cells for RT-PCR. The I420R substitution in gp120 is a CoRbs knockout mutation.