Table 1.
Inferred MAb IgG gene family usagea
| MAb | Genes | CDR3 length (aa) | Mutation frequency (%) | Note | ||
|---|---|---|---|---|---|---|
| Heavy chain | VH family | D family | JH family | VH | ||
| VcT16 | IGHV3-7*01 | IGHD3-10*01 | IGHJ6*02 | 18 | 8 | |
| VcT39 | IGHV1-2*02 | IGHD3-16*01b | IGHJ6*02 | 25 | 7 | |
| Vc813 | IGHV1-2*02 | IGHD3-16*01b | IGHJ6*02 | 25 | 6 | |
| Vc822 | IGHV1-69*09 | IGHD3-22*01 | IGHJ3*02 | 22 | 11 | |
| Vc827 | IGHV4-4*07 | IGHD3-10*01 | IGHJ4*02 | 15 | 14 | |
| Vc908 | IGHV1-69*01 | IGHD1-26*01 | IGHJ6*03 | 25 | 5 | |
| Vc932 | IGHV1-69*01 | IGHD3-22*01 | IGHJ6*03 | 23 | 7 | |
| Vc949 | IGHV1-69*01 | IGHD4-17*01 | IGHJ6*03 | 19 | 6 | |
| VRC06 | IGHV1-2*02 or IGHV1-2*04 | IGHD4-17*01b | IGHJ1*01 | 17 | 29 | 21-bp insertion in FR3 |
| VRC06b | IGHV1-2*02 or IGHV1-2*04 | IGHD4-17*01b | IGHJ1*01 | 17 | 32 | 21-bp insertion in FR3 |
| VRC03 | IGHV1-2*02 or IGHV1-2*04 | IGHD3-22*01b | IGHJ1*01 | 16 | 29 | 21-bp insertion in FR3 |
| Light chain | VK family | JK family | VK | |||
| VcT16 | IGKV3-11*01 | IGKJ3*01 | 10 | 2 | ||
| VcT39 | IGKV1-39*01 or IGKV1D-39*01 | IGKJ4*01 | 8 | 5 | ||
| Vc813 | IGKV1-39*01 or IGKV1D-39*01 | IGKJ4*01 | 8 | 6 | ||
| Vc822 | IGKV1-5*03 | IGKJ1*01 | 9 | 3 | ||
| Vc827 | IGKV1-13*02 or IGKV1D13*01 | IGKJ4*03 | 9 | 11 | ||
| Vc908 | IGKV3-15*01 | IGKJ2*01 | 10 | 2 | ||
| Vc932 | IGKV3-20*01 | IGKJ3*01 | 9 | 1 | ||
| Vc949 | IGKV4-1*01 | IGKJ1*01 | 9 | 4 | ||
| VRC06 | IGKV3-20*01c | IGKJ2*01 | 5 | 16 | 6-bp deletion in CDR1 | |
| VRC06b | IGKV3-20*01c | IGKJ2*01 | 5 | 18 | 6-bp deletion in CDR1 | |
| VRC03 | IGKV3-20*01c | IGKJ2*01 | 5 | 20 | 6-bp deletion in CDR1 | |
a
JoinSolver (38) and IMGT were used for determining the gene utilization in this table.
b
Putative D segments assigned with less than 95% confidence due to the small number of consecutively matching residues compared to the V-to-J distance.
c
IGKV3-20*01 was selected over IGKV3-NL5 in order to use genes mapped to the IGK locus on chromosome 2.