Fig 1.

Gating strategy for and CD4 mRNA expression in T cell populations sorted from multiple anatomic compartments in chronically SIV-infected AGMs. (A) After gating on singlets and the lymphocyte populations on forward and side scatter plots, CD3⁺ T lymphocytes were first separated into CD4⁺, CD8⁺, and CD4⁻ CD8⁻ (DN) populations. The DN T lymphocytes were collected without further subdivision, whereas the CD4⁺ lymphocyte population was subdivided into CD95⁺ (memory) and CD95⁻ (naïve) populations prior to collection. CD95⁺ (memory) cells of the CD8⁺ lymphocyte population were also collected. Postsort purities determined on the PBMC fractions were routinely >98%. CD4 RNA copy numbers are shown per 10⁶ cells in panel B for CD4⁺ CD95⁺, panel C for CD4⁺ CD95⁻, panel D for DN, and panel E for CD8⁺ CD95⁺ T lymphocyte populations isolated from multiple anatomic compartments of SIV-infected AGMs. mes LN, mesenteric lymph nodes; Ax LN, axillary LNs; Spl, spleen. Horizontal lines represent medians.