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. 2012 Oct;194(19):5285–5293. doi: 10.1128/JB.00840-12

Fig 5.

Fig 5

Immunoblot analysis of H-NS66 in the presence or absence of ssrA. (A) Total cell extracts of the strains ZK819-66 (lane 1) and ZK819-66ΔssrA (lane 2) were probed using polyclonal anti-H-NS antibody. Anti-RRF antibody was used to detect the loading control RRF. (B) Wild-type H-NS in cell extracts of ZK819 (lane 1) and H-NS66 in ZK819-66ΔssrA (lane 2). (C) H-NS and HNS66 in total cell extracts from different strain with or without plasmid-encoded SsrADD. Wild-type H-NS was detected in the presence or absence of SsrADD (lanes 1 and 5). H-NS66 was detected in the presence or absence of SsrA (lanes 2 and 3). H-NS66 was detected in the presence of SsrADD (lanes 4 and 6) (n = 2).