Fig 5.
Fatty acid composition analysis of P. aeruginosa strains fed long-chain perdeuterated fatty acids. (A) Gas chromatograms with FID detection of FAME extracts obtained from stationary-phase cultures of P. aeruginosa grown in liquid LB-BSA medium supplemented with either 100 μg/ml of perdeuterated tetradecanoate (d27-C14:0) or hexadecanoate (d31-C16:0). Asterisks indicate deuterated FAMEs of identical structure to those already assigned in Fig. 3 based on time of elution and mass spectra analysis. FAME peaks unique to d31-C16:0-fed cultures are indicated (○). (B) Mass spectra analysis of the d31-C16:0-specific FAME with the parent molecular ion ([M]+) and diagnostic fragment ions labeled, including for the uniformly deuterium-labeled McLafferty ion (m/z = 77).