Fig 4.

Relative amounts of the two plasmid types generated after transfer of the indicated 2xoriT plasmid. DNAs were extracted from pooled transconjugant cells after matings done in triplicate and digested with XmnI and EcoO109I, and the fragments were assayed by 1.8% agarose gel electrophoresis. The smaller fragment is derived from plasmids initiating transfer at oriT(1) and terminating at oriT(2); the larger fragment is derived from plasmids initiating and terminating at the same locus. In each panel, only the part of the gel containing the bands relevant for the analysis is shown.