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Expression level of mutant MutL proteins in vivo. An immunoblot with the relative level of each mutant MutL protein in vivo is shown. (A and B) Whole-cell extracts probed for MutL or as a loading control DnaN on a duplicate blot prepared from the same extract are shown. The mutL+ represents the wild-type mutL allele expressed from amyE with 1 mM IPTG, the same expression condition used for each mutant protein tested.
