Table 3.
Missense mutations in B. subtilis mutL cause defects in MMRa
| Genotype | Mutation rate × 10−9b | Relative mutation rate | Mutations per culture |
|---|---|---|---|
| Wild type (PY79) | 3.9 (2.0–5.8) | 1.0 | 2.5 |
| amyE::Pspac-mutL+ | 3.8 (2.0–5.6) | 1.0 | 2.3 |
| mutL::spc | 192.1 (184.1–200.1) | 50.1 | 117.2 |
| mutL(A17V) | 180.1 (173.1–187.2) | 47.0 | 126.6 |
| mutL(G18A) | 35.7 (29.9–41.4) | 9.3 | 19.8 |
| mutL(P24L) | 81.0 (74.8–87.2) | 21.1 | 54.1 |
| mutL(E30A) | 139.6 (132.0–147.2) | 36.4 | 83.4 |
| mutL(N34H) | 177.0 (169.3–184.8) | 46.2 | 110.3 |
| mutL(S40A) | 149.0 (142.5–155.4) | 38.9 | 112.3 |
| mutL(N60S) | 2.5 (0.9–3.9) | 0.7 | 1.4 |
| mutL(G63R) | 145.9 (137.3–154.5) | 38.1 | 76.1 |
| mutL(K80E) | 150.7 (142.8–158.5) | 39.3 | 89.2 |
| mutL(G94S) | 160.7 (153.5–168.0) | 41.9 | 107.0 |
| mutL(S102R) | 24.9 (20.1–29.7) | 6.5 | 15.3 |
| mutL(R176G) | 35.0 (30.3–39.6) | 9.1 | 26.2 |
| mutL(G234V) | 98.1 (91.5–104.6) | 25.6 | 65.0 |
| mutL(R256C) | 141.5 (134.0–148.9) | 36.9 | 87.4 |
a
The mutation rate, the relative mutation rate, and the mutations conferred per culture for each mutL allele examined are shown. Mutation rates and mutations per culture (17) were calculated by FALCOR (Fluctuation AnaLysis CalculatOR) using the MSS-MLE method (23). Calculations are based on data collected through rifampin resistance by fluctuation analysis for 14 to 50 independent cultures per strain. The relative mutation rate reflects normalization of mutation rates to that obtained for Pspac mutL+.
b
The error shown in the parentheses reflects the 95% confidence interval.