Table 2.
Plasmids
| Plasmid | Relevant characteristic(s) | Reference |
|---|---|---|
| pZep08 | GFP reporter plasmid; Apr Cmr | 31 |
| pBAD33 | Vector with l-arabinose-inducible PBAD promoter; Apr | 6 |
| pBADvirB | virB gene expressed from l-arabinose-inducible PBAD promoter; Apr | 6 |
| pZep-virB-310 | Positions −310–+20 of PvirB cloned into pZep08 | This study |
| pZep-virB-310 SDM | pZep-virB-310 with putative VirB binding site mutated | This study |
| pZep-virB-150 | Positions −150–+20 of PvirB cloned into pZep08 | This study |
| pZep-virB-80 | Positions −80–+20 of PvirB cloned into pZep08 | This study |
| pZep-virB-80-icsB | Positions −240–−80 of PicsB cloned into pZep-virB-80 | This study |
| pZep-virF-380 | Positions −380–+64 of PvirF cloned into pZep08 | This study |
| pZep-virF-280 | Positions −280–+64 of PvirF cloned into pZep08 | This study |
| pZep-virF-280 SDM | pZep-virF-280 with putative virF binding site mutated | This study |