Table 3.
Primers
| Oligonucleotide | Sequence (5′–3′)a |
|---|---|
| icsB fw.TAG | TGTACCTCGTGAGCATATGTAGT |
| icsB rev.TAG | GGGGCATTGATTGCAGTTTT |
| virB.rev.+190.XbaI | TTTTCTAGATTTTGGTTGACGAAGGTTAAATC |
| virB.fw.-80.NotI | TTTGCGGCCGCTTGCATCAATCCAGCTATTAAA |
| virB.fw.-310.NotI | TTTGCGGCCGCGCCGATTCTCTTTCTCTGATTC |
| virB.RT.rev | TTTTGGTTGACGAAGGTTAAATC |
| virB.RT.fw.+2 | ACTGCATTTAACTTTGTCAATA |
| virB.fw.SDM | GATTCTCTTTCTCTGgctgggcGCTGGATATGATTTAG |
| virB.rev.SDM | CTAATCATATCCAGCgcccagcAGAGAAAGAGAATC |
| virF.rev.+160.XbaI | TTTTCTAGAAATGACGGTTAGCTCAGGCA |
| virF.fw.-280.NotI | TTTGCGGCCGCTGGAGCCTCCAGTCTGAAG |
| virF.fw.-380.NotI | TTTGCGGCCGCCTTCACGATCGCAATATGGA |
| virF.RT.fw | GGTTCGCTTGCATAACTATA |
| virF.RT.rev | ATAAAAGCAATTTGCCCTTC |
| virF.fw.SDM | TATGGTTATAGTCCCgggCAGTGCgggcACTTAGCTTGTTGCA |
| virF.rev.SDM | TGAACAAGCTACGTgcccGCACTGcccGGGACTATAACCATA |
| pZec-6FAM.F | FAM-ACAAATCCGCCCTCTAGCAGCCCG |
| pZec-confirm.R | ACGGGAAAAGCATTGAACAC |
a
Lowercase letters in sequences represent bases introduced during site-directed mutagenesis. Underlining indicates the locations of recognition sequences for restriction enzymes.