Fig 1.

The thermosensitive strain highlights a PdhS loss of function. (A) Wild-type (WT) strain of B. abortus and the thermosensitive (Ts) strain grown at the permissive temperature (36°C) or restrictive temperature (42°C), with a plasmid carrying the wild-type pdhS sequence (rescue plasmid) or the empty vector. (B) Sequence of the 15-bp scar at the insertion site of Tn4430. (C) DivK-YFP localization in the B. abortus TS strain. Differential interference contrast (DIC) images are shown in the upper panels, and YFP fluorescence is shown in the bottom panels. White arrows show polar DivK-YFP. The percentage in each panel indicates the proportion of bacteria in which a polar DivK-YFP was detected. Scale bar, 2 μm.