Fig 1.
Influence of the AbrB2 regulator on growth of Synechocystis under standard conditions. (A) Schematic representation of the abrB2 chromosome locus in the WT strain (see CyanoBase) and the abrB2-deleted mutant (ΔabrB2::Kmr) constructed in this study. The genes are represented by boxes pointing in the direction of their transcription. The PCR primers specific to the slr0846 and sll0823 genes are represented by the small gray triangles, and the sizes of the products they generated upon amplification of the abrB2 chromosome locus of the WT (1,104 bp) and abrB2-deleted (ΔabrB2::Kmr; 1,966 bp) chromosomes are indicated by double arrows. (B) UV light image of the agarose gel, showing the 1,104-bp and 1,966-bp PCR product typical of the WT and abrB2-deleted chromosomes, respectively; the results show that the abrB2-deleted mutant harbors no WT copies of the chromosome. (C) Growth of the WT strain and the ΔabrB2 mutant (this experiment was repeated three times and yielded statistical deviations too small to be represented).