TABLE 1.
Characteristics of plasmids used in this study
| Plasmid use and name | Relevant feature(s) | Reference |
|---|---|---|
| Targeted deletion of abrB2 in Synechocystis | ||
| pGEMT | AT overhang Ampr cloning vector | Promega |
| pUC4K | Source of Kmr marker gene | Pharmacia |
| pΔsll0822 | pGEMT with Synechocystis sll0822 flanking sequences, with sll0822 coding sequence (from bp 7 to 384) replaced by SmaI site | This study |
| pΔsll0822::Kmr | pΔsll0822 with Kmr marker inserted into unique SmaI site | This study |
| High-level expression of abrB2 in Synechocystis | ||
| pFC1 | Replicating plasmid for heat-inducible gene expression in Synechocystis | 32 |
| pSll0822 | pFC1 with sll0822 CS cloned between NdeI-EcoRI sites | This study |
| Analysis of hox and abrB2 promoters in Synechocystis | ||
| pSB2A | Kmr Smr/Spr replicative promoter probe plasmid harboring unique SnaBI site in front of its promoterless cat reporter gene | 27 |
| pPS1 | Kms derivative of pSB2A generated after cleavage with NsiI and religation | This study |
| pJD1 | hox PRa (bp −794 to +173 relative to TSS) cloned at SnaBI site of pPS1 | This study |
| pJD2 | hox PR (bp −522 to +173 relative to TSS) cloned at SnaBI site of pPS1 | This study |
| pJD3 | hox PR (bp −403 to +173 relative to TSS) cloned at SnaBI site of pPS1 | This study |
| pJD4 | hox PR (bp −170 to +173, relative to TSS) cloned at SnaBI site of pPS1 | This study |
| pPS2 | abrB2 PR (bp −75 to +88 relative to TSS) cloned at SnaBI site of pSB2A | This study |
| pPS2 m-8 | pPS2 harboring T→G mutation at −8 position relative to TSS | This study |
| pPS2 m-13 | pPS2 harboring T→G mutation at −13 position relative to TSS | This study |
| pPS2 m-15 | pPS2 harboring T→G mutation at −15 position relative to TSS | This study |
| pPS2 m-4746 | pPS2 harboring TT→GG mutation at positions −47 to −46 relative to TSS | This study |
| Production of AbrB2 in E. coli | ||
| pET14b | Ampr E. coli plasmid for production of 6×His-tagged proteins | Novagen |
| pSS1 | pET14b plasmid with sll0822 CS cloned between NdeI and BamHI sites | This study |
a
PR, promoter region.