Fig 4.

Growth characteristics and complementation of the P. aeruginosa ΔPA5174 strain. (A) Growth of the P. aeruginosa PAO1 parent strain (●), ΔPA5174 strain (■), PA5174 back-complemented with plasmid (TMT41 [△]), and the E. coli FabH-complemented strain (TMT38 [□]) in LB at 37°C. Basal expression of PA5174 on the plasmid is sufficient for full complementation in the absence of IPTG induction. (B) The rate of radiolabeled precursor incorporation by FAS ([³H]acetate [black bars]) and RNA ([³H]uridine [white bars]) biosynthetic pathways was measured after a 30-min incubation period in supplemented M9 minimal medium. The percent incorporated label was normalized to the value of the wild-type (Wt) P. aeruginosa PAO1 control. Error bars represent the standard deviations from the means calculated from three independent experiments.