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Siderophore secretion in P. aeruginosa ΔPA5174. The wild-type P. aeruginosa PAO1 (Wt), TMT39 (ΔPA5174), ΔPA5174(pZEN-PA5174) (TMT41), and ΔpqsD PQS− (TMT02) strains were streaked onto LB agar plates containing CAS and incubated at 37°C for 24 h. Zones of yellow-orange clearing indicate sequestration of Fe3+ away from CAS- Fe3+ complexes by secreted siderophores.
