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SCVs emerge during exponential-phase NCP growth. TSB was inoculated with a low number of S. aureus SH1000 bacteria (to reduce the probability of SCVs being present in the inoculum), and total and SCV CFU was measured over time. Data points represent the averages of 8 independent cultures. Error bars represent the standard deviations of the means. The limit of detection was 10 CFU ml−1.
