Table 2.
Strain | Versionc of: |
UV survival | Amt of SOS expression |
Rel. recb | ||
---|---|---|---|---|---|---|
recA | radA | RFI | % >9-fold (18-fold) | |||
SS996 | + | + | 0.91 ± 0.3 | 32.8 | 98.2 (36.4) | 1.00 ± 0.2 |
SS8253 | + | op | 0.92 ± 0.4 | 31.2 | 97.2 (32.3) | 1.12 ± 0.3 |
SS9023 | 4142 | + | NDd | 34.8 | 99.3 (41.3) | ND |
SS8254 | 4142 | op | ND | 32.6 | 98.9 (31.3) | ND |
Data are the averages of three experiments or the counting of 800 to 1,000 cells. For all three experiments, the cells were grown in minimal media at 37°C into log phase before the treatment. The UV survival reported is survival after 20 J/m2 of irradiation. For the SOS test, cells were irradiated at 10 J/m2, imaged after 1.5 h of incubation at 37°C in the dark, and quantified as described in Materials and Methods.
Rel. rec., relative recombination. Recombination was measured by inheritance of a marker after P1 transduction. An equal titer of P1 lysate (multiplicity of infection of 0.1) was used for each strain.
Abbreviations are as for Table 1.
ND, not determined.