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. 2012 Oct;194(19):5325–5333. doi: 10.1128/JB.00674-12

Table 2.

Summary of phenotypes in the radA overexpression strain relative to a wild-type straina

Strain Versionc of:
UV survival Amt of SOS expression
Rel. recb
recA radA RFI % >9-fold (18-fold)
SS996 + + 0.91 ± 0.3 32.8 98.2 (36.4) 1.00 ± 0.2
SS8253 + op 0.92 ± 0.4 31.2 97.2 (32.3) 1.12 ± 0.3
SS9023 4142 + NDd 34.8 99.3 (41.3) ND
SS8254 4142 op ND 32.6 98.9 (31.3) ND
a

Data are the averages of three experiments or the counting of 800 to 1,000 cells. For all three experiments, the cells were grown in minimal media at 37°C into log phase before the treatment. The UV survival reported is survival after 20 J/m2 of irradiation. For the SOS test, cells were irradiated at 10 J/m2, imaged after 1.5 h of incubation at 37°C in the dark, and quantified as described in Materials and Methods.

b

Rel. rec., relative recombination. Recombination was measured by inheritance of a marker after P1 transduction. An equal titer of P1 lysate (multiplicity of infection of 0.1) was used for each strain.

c

Abbreviations are as for Table 1.

d

ND, not determined.