Fig 5.

CP footprinting of ToxT on PctxAB. Toxbox 1 and toxbox 2 are indicated by solid arrows with the correlating numerical position from the transcriptional start site. “C,” “T,” “A,” and “G” at the top left refer to the nucleotide lanes of the sequencing ladder. The dotted arrows and numbers to the left show the locations of the GATTTTT repeats, and the solid arrow indicates the position of the vector. The solid lines and black dots to the right of the toxboxes indicate the locations of ToxT-dependent transcriptional defects identified in the single-point-mutagenesis experiments. This footprint was created by using the pJW211 construct, which extends to position −76.