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EMSA using purified recombinant S-PhoP with various mutants of the amtB promoter. The probes used are as described for Fig. 3A, and the amounts of PhoP protein used are indicated. Three PhoP/DNA complexes are indicated by arrows. The gel was run in ice-cooled 1× Tris-glycine-EDTA (TGE) buffer at 14 V/cm for about 50 min.
