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Competitive EMSA of S. coelicolor amtB promoter with purified recombinant His-GlnR and S-PhoP. The 101-bp DNA fragment of the amtB promoter region (oWT, 0.04 pmol) was used for incubation with various amounts of GlnR and PhoP. The protein-DNA complexes are indicated. The gel was run in ice-cooled 1× TGE buffer at 14 V/cm for about 2 h.
