Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype and/or descriptiona | Source or reference |
|---|---|---|
| Plasmids | ||
| pIJ8660 | Promoter probe vector containing the egfp gene as indicator; oriT RK2 attP aac(3)IV | 20 |
| pIJ8660-hrdBp | 896-bp PCR fragment of hrdB promoter region inserted into the KpnI-BamHI site of pIJ8660 | 16 |
| pIJ8660-amtBpWT | 500-bp PCR fragment of the wild-type amtB promoter region inserted into the BamHI-NdeI site of PIJ8660 | This study |
| pIJ8660-amtBp3 | a3-b3 site mutation within the amtB promoter region from PIJ8660-amtBpWT | This study |
| pIJ8660-amtBp1 | a1-b1 site mutation within the amtB promoter region from PIJ8660-amtBpWT | This study |
| pIJ8660-amtBp2 | a2-b2 site mutation within the amtB promoter region from PIJ8660-amtBpWT | This study |
| pET28a | Expression vector carrying N-terminal His tag; Kmr | Novagen |
| pEXSCR | pET28a carrying the S. coelicolor glnR gene | 23 |
| pET32a | Expression vector carrying the N-terminal Trx-His-S tag; Ampr | Novagen |
| pET32a-PhoP | pET32a carrying the S. coelicolor phoP gene | This study |
| Strains (S. coelicolor) | ||
| M145 | Wild type, SCP1− SCP2− | John Innes Centre |
| M145/pIJ8660 | M145 integrated with pIJ8660 | This study |
| SCamtB-WT | M145 integrated with pIJ8660-amtBpWT | This study |
| SCamtB-3 | M145 integrated with pIJ8660-amtBp3 | This study |
| SCamtB-1 | M145 integrated with pIJ8660-amtBp1 | This study |
| SCamtB-2 | M145 integrated with pIJ8660-amtBp2 | This study |
| M145/pIJ8660-hrdBp | M145 integrated with pIJ8660-hrdBp | 17 |
a
Ampr, ampicillin resistance; Kmr, kanamycin resistance.