Fig 5.

Reduction of total BCFA through butyrate supplementation decreases resistance to LL-37 and intracellular CFU of WT L. monocytogenes. WT bacteria were grown in the presence of butyrate to enrich for straight-chain FAs. Survival of WT bacteria grown in BHI or in BHI + 250 mM sodium butyrate was determined by measuring CFU after on hour of exposure to antimicrobial peptide LL-37 (A) or between 0.5 and 2 hpi in RAW 264.7 macrophages (B). The data plotted represent averages of three independent experiments, with error bars denoting the standard deviation. Each independent experiment was performed with triplicates. Significant differences are indicated by asterisks (**, P < 0.01).