Table 2.

FA composition in L. monocytogenes can be manipulated through genetic mutation or metabolic supplementation^a

FA	FA composition (%)
	WT L. monocytogenes				BKD− L. monocytogenes
	BHI	BHI + 2MB	BHI + butyrate	BHI + Leu	BHI	BHI + 2MB
Straight FAs
12:0	0.09	0.09	3.07	0.17	0.44	0.08
14:0	0.27	0.16	41.15	0.49	15.46
16:0	0.69	0.53	24.39	0.45	43.88	3.02
18:0			0.17		1.83
Total	1.05	0.78	68.78	1.11	61.61	3.1
Even iso FAs
14:0 iso	0.45	0.26	1.72	0.85	10.81	0.61
16:0 iso	2.19	1.38	1.29	2.76	18.23	0.32
18:0 iso					0.52
Total	2.64	1.64	3.01	3.61	29.56	0.93
Odd iso FAs
13:0 iso			0.17	0.24
15:0 iso	8.11	5.94	5.11	44.78	0.80
17:0 iso	2.43	1.84	0.55	12.25	0.32
Total	10.54	7.78	5.83	57.27	1.12	0
Anteiso FAs
13:0 anteiso	0.16	0.16	0.24	0.08		0.19
15:0 anteiso	51.82	53.65	18.84	24.48	3.56	57.1
17:0 anteiso	33.5	35.55	2.55	12.65	1.63	38.14
19:0 anteiso	0.08	0.07		0.05		0.09
Total	85.56	89.43	21.63	37.26	5.19	95.52

^aBHI, brain heart infusion medium; 2MB, 5 mM 2-methylbutyric acid; butyrate, 250 mM sodium butyrate; Leu, 100 mM leucine. FA analysis was performed with bacteria harvested from overnight cultures by Microbial ID.