Table 3.

Doubling times of L. monocytogenes intracellular growth in RAW 264.7 macrophages with or without leucine supplementation^a

Time (h) postinfection	Mean doubling time (min) ± SD		P
	No supplementation	With 100 mM leucine
0.5–3	162.7 ± 11.0	861.8 ± 3,019.4	0.675
3–5	43.8 ± 5.7	99.5 ± 18.3	0.006
5–7	30.8 ± 2.6	55.7 ± 7.6	0.005
7–12	65.5 ± 9.1	100.5 ± 8.8	0.001

^aDoubling times were calculated for the growth curves shown on Fig. 6C using the following equation: time elapsed/[(log₁₀ second OD value − log₁₀ first OD value)/log₁₀ 2]. The values shown here are averages of triplicates in a single experiment but represent three independent experiments. The P values were calculated between each pairwise comparison by unpaired Student t test.