Table 1.
C. acetobutylicum strains, plasmids, and primers used in this study
| Strain, plasmid, or primer | Description or sequencea (5′→3′) | Source or reference |
|---|---|---|
| C. acetobutylicum strains | ||
| ATCC 824 | Wild type | ATCC |
| xfp::intron | xfp-inactivated mutant, Emr | This study |
| 824-pIMP1 | Strain carrying pIMP1 | |
| 824-pIMP1XFP | xfp-overexpressed strain, Emr | This study |
| Plasmids | ||
| pWJ1 | Group II intron | 44 |
| pWJ1-xfp | pWJ1 derivative for inserting intron into xfp | This study |
| pIMP1 | Ampr Emr | 29 |
| pIMP1-xfp | pIMP1 derivative carrying CAC1343 gene | This study |
| pET28a | Novagen | |
| pET28a-xfp | pET28a derivative carrying CAC1343 gene | This study |
| Primers | ||
| xfp-IBS | AAAACTCGAGATAATTATCCTTACACTACAGGTTCGTGCGCCCAGATAGGGTG | |
| xfp-EBS1d | CAGATTGTACAAATGTGGTGATAACAGATAAGTCAGGTTCCATAACTTACCTTTCTTTGT | |
| xfp-EBS2 | TGAACGCAAGTTTCTAATTTCGGTTTAGTGTCGATAGAGGAAAGTGTCT | |
| xfp-UNI | CGAAATTAGAAACTTGCGTTCAGTAAAC | |
| pIMP1-xfp_F | CGGGATCCATGCAAAGTATAATAGGAAAAC | |
| pIMP1-xfp_R | TCCCCCCGGGTTATACATGCCACTGCCAATTAG | |
| pET28a-xfp_F | AAGCTTATGCAAAGTATAATAGGAAAACATAAGG | |
| pET28a-xfp_R | GGATCCTTATACATGCCACTGCCAATTAGTT |
a
The introduced restriction sites are underlined.