Fig 3.

Detection of cytokines in brain tissue at various times after scrapie infection. Protein levels of 9 cytokines in the brains of scrapie 22L-infected mice were measured by multiplex assays at various days postinoculation. The six cytokines for which results are shown had values significantly elevated over those for normal control mice (N) at various days postinoculation. Data for CCL2 were obtained from samples assayed at a 4% brain concentration, and other cytokines were assayed at a 1% brain concentration. IL-9, IL-13, and gamma interferon were also analyzed, but no values above those for normal controls were detected. Two to four mice per group were studied. Statistical analysis was carried out using one-way ANOVA with GraphPad Prism software. ***, P < 0.001; **, P < 0.01; *, P < 0.05.