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Transcription of ac34 in Sf9 cells infected with AcMNPV. (A) RT-PCR analysis of ac34 transcripts. Total RNA was isolated from AcMNPV-infected Sf9 cells at the time points indicated above the lanes. (B) 5′ RACE analysis of the ac34 transcriptional start sites. The early promoter motif TTCTG and the late promoter motif ATAAG are boxed. The arrows indicate the locations of the ac34 mRNA transcription initiation sites.
