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Temporal expression analysis of Ac34. Sf9 cells were infected with vHA:Ac34 at an MOI of 5 TCID50/cell. The cells were harvested at 6, 12, 24, 48, 72, and 96 h p.i., and samples were subjected to immunoblotting. The membrane was probed with anti-HA antibody to detect HA:Ac34. An anti-actin antibody was used as a loading control.
