Fig 5.

Fast and unidirectional movement of AAV2 toward the perinuclear region. Videos were taken at 1 h after pulse infection with Cy5-AAV2 using an Olympus IX81 inverted fluorescence microscope. (A) Snapshots of consecutive frames with a 2.2-s time interval show the fast and directed movement of Cy5-AAV2 (purple and indicated by arrowheads) on microtubules (green) toward the perinuclear region. (B) Trajectory of the Cy5-AAV2 particle indicated by arrowheads in panel A. Frames subject to directional transport are shown as green points and lines, and those with confined motion in the perinuclear region are indicated as black points. The bar represents 5 μm. (C) Instantaneous velocity of the particle (indicated by arrowheads in panel A) over time. (D) The displacement from the starting position (panel a) and mean square displacement (MSD) (panel b) are plotted for the particle indicated by arrowheads in panel A. In panel b, the plot between MSD and time interval (ΔT) is fitted by a quadratic curve (dashed line, MSD = 0.7269t² + 1.902t) with r² = 0.9997.